News from this website 4month5Japan, an authoritative international journal《Nature.Communication》(Nature Communications）Published online by the team of Professor Wang Jianxin from the School of Computing at bet365 login games South University and Luo Feng from Clemson University in the United StatesProfessor,Sun Yat-sen bet365 login gamesZhongshan Eye CenterXiao ChuanleAssociate ResearcherThe latest research results of cooperation“De novo diploid bet365 login games based on long reads (De novo diploid genome bet365 login games using long noisy reads）”。The paperProposed a new diploid bet365 login games method based on third-generation sequencing data，And developed the corresponding softwarePECAT.School of Computer Science, bet365 login games South UniversityNie Fan,Ni PengCommon to the paperThe first author is Professor Wang Jianxin from the School of Computer Science, bet365 login games South UniversityPaperCo-corresponding author，bet365 login games South University is the first signing unit。This research is supported by the National Key Research and Development Program、National Natural Science Foundation of China、Supported by multiple projects such as Xiangjiang Laboratory’s unveiling project。
Third generation sequencing technology (Oxford Nanopore sequencing andRapid advances in PacBio single-molecule real-time sequencing) produce longer and more accurate reads，Brings new opportunities and challenges to genome bet365 login games research。For diploid bet365 login games，The third generation reads still contain higher sequencing errors，It is difficult for bet365 login games algorithms to distinguish sequencing errors from haplotype difference information，Thus generating bet365 login games results of mixed haplotypes，It contains a large number of haplotype switching errors, And a large portion of genetic information is lost。
In response to this limitation，Professor Wang Jianxin’s team and others recently published in In research paper from Nature Communications，Through in-depth analysis of the differences between third-generation long reads carrying sequencing errors and haplotype differences，Proposed a long read error correction algorithm that preserves haplotype difference information，Prevent haplotype difference information from being removed as sequencing errors，Ensures the consistency of haplotype difference information，The haplotype consistency of its error-corrected readings can reach 99.4%。On this basis, a diploid bet365 login games algorithm based on local haplotype clustering was designed，bet365 login games results to achieve haplotype mixing in the first round of bet365 login games。In the second round of bet365 login games，Align reads to hybrid bet365 login games results，Identifies the single nucleotide polymorphism (SNP) information carried by the read，Identify overlapping relationships with inconsistent haplotypes through local clustering，Filter inconsistent overlapping relationships and assemble again to achieve haplotype bet365 login games results。On multiple test data，The method PECAT proposed in the paper obtains more continuous haplotype bet365 login games results。Among them，On Bull Data for Nanopore R9，PECAT achieves bet365 login games results with nearly resolved haplotypes。And on the human HG002 sample nanopore R10 data，PECAT achieved a haplotype continuity index (phase block NG50) of 59.4/58.0Mb bet365 login games result。
PECAT bet365 login games algorithm framework diagram
First instance: Yu Tao Second instance: Deng Haodi Third instance: Li Yin
